World Pathology Week

Biography

Dr Miriam S Eden has completed MD Pathology from CMC, Vellore, MGRU University in year 2016 and presently is a Head section in Department of  Pathology, Wanless Hospital Miraj Medical Centre, Miraj, Maharashtra, India..  Presently has three publications at hand.

Abstract

This is the study about the use of   p53 as a prognostic marker to detect early lymph node metastasis in carcinoma of penis so as to avoid unnecessary lymphadenectomy.   Amputed specimens of penis were studied using p53 immuno marker, and correlation  with the histopathological variables and clinical stage was done. It was a retrospective study  of 49 cases of partial /total amputation with lymphadenectomy of penile cancers  were divided into metastatic and non metastatic groups on H&E sections in which 16/49 had metastasis. The slides were processed for  immunohistochemical technique using anti‑p53 antibodies.  The p53 tumor density was calculated in 100 cells with 20% cut off and  was graded as positive when more than 20% of the tumor cells with positive  nuclear staining.   All the pathological and clinical variables were calculated by Chi square test.  Results showed that 13 /49 cases showed increased p53 tumor density, pvalue of 0.05 was considerd significant in our study.  P53 did not correlate statistically with histopathological and clinical variables like phimosis, BXO, clinical node status, tumor type, tumor grade, depth of invasion, LVI.  P53 had low positive predictive value. Therefore in our study p53 cannot predict the early lymph node metastasis because of low positive predictive value, even though they are sensitive.

 Therefore   P53 Immunomarker had low specificity and low positive predictive value, which suggest that it is not able to predict early lymph node metastasis even though it is sensitive and thus it is not helpful to decide for prophylactic lymphadenectomy.